The Rate of Mutation of L5178Y Asparagine-dependent Mouse Leukemia Cells to Asparagine Independence and Its Biological Consequences1
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چکیده
LSI78Y mouse lymphoblastic leukemia cells normally require asparagine (ASN) for growth, but phenotypically stable ASN-independent cells are detectable at low fre quency by direct cloning, in ASN-free soft-agar medium, of cells from cultures that have been grown nonselectively in medium containing ASN. The rate of mutation in vitro to ASN independence was determined by the Luria-Delbruck fluctuation test to be approximately 9 x 10~'/cell/genera
منابع مشابه
The rate of mutation of L5178Y asparagine-dependent mouse leukemia cells to asparagine independence and its biological consequences.
LSI78Y mouse lymphoblastic leukemia cells normally require asparagine (ASN) for growth, but phenotypically stable ASN-independent cells are detectable at low fre quency by direct cloning, in ASN-free soft-agar medium, of cells from cultures that have been grown nonselectively in medium containing ASN. The rate of mutation in vitro to ASN independence was determined by the Luria-Delbruck fluctua...
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